ELISA試劑盒實驗操作乙醇脫氫酶6抗體的原理

ELISA試劑盒是酶聯接免疫吸附劑測定( Enzyme-Linked Immunosorbnent Assay )的簡稱。它是繼免疫熒光和放射免疫技術之后發展起來的一種免疫酶技術。此項技術自70年代初問世以來，發展十分迅速，目前已被廣泛用于生物學和醫學科學的許多領域。
英文名稱  Anti-ADH6 
中文名稱  乙醇脫氫酶6抗體 
別    名  ADH 5; ADH 6; Adh6; ADH6_HUMAN; Alcohol dehydrogenase 6 (class V); Alcohol dehydrogenase 6; Aldehyde reductase; Class V alcohol dehydrogenase 6; UDP GLUCURONOSYLTRANSFERASE 2B10 PRECURSOR, MICROSOMAL. 
濃    度  1mg/1ml 
規 格  0.2ml/200μg  
抗體來源  Rabbit  
克隆類型  polyclonal 
交叉反應  Human, Mouse, Rat, Pig, Cow  
產品類型  一抗    
研究領域  腫瘤 細胞生物 信號轉導 新陳代謝  
蛋白分子量  predicted molecular weight: 39kDa 
性    狀  Lyophilized or Liquid 
免 疫 原  KLH conjugated synthetic peptide derived from human ADH6 
亞    型  IgG 
純化方法  affinity purified by Protein A 
儲 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 
產品應用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500 
（石蠟切片需做抗原修復） 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
產品介紹 ADH6 (alcohol dehydrogenase 6), also known as ADH-5, is a 368 amino acid member of the class V zinc-containing alcohol dehydrogenase family. This family of enzymes functions to metabolize a wide variety of substrates such as retinol, hydroxysteroids, ethanol, aliphatic alcohols and lipid peroxidation products. Localized to the cytoplasm and expressed in the stomach and liver, ADH6 catalyzes the reversible oxidation of alcohols to their corresponding aldehydes or ketones and is able to bind two zinc ions as cofactors. ADH6 contains a glucocorticoid response element upstream of its 5' UTR which is thought to be a steroid binding site, suggesting that expression of ADH6 may be under hormonal control. Multiple isoforms of ADH6 exist due to alternative splicing events.
Subcellular Location : Cytoplasm.
Tissue Specificity : Stomach and liver.
Similarity : Belongs to the zinc-containing alcohol dehydrogenase family. Class-V subfamily.
Database links : UniProtKB/Swiss-Prot: P28332.2
ELISA試劑盒正式試驗時，應分別以陽性對照與陰性對照控制試驗條件，待檢樣品應作一式二份，以保證實驗結果的準確性。有時本底較高，說明有非特異性反應，可采用羊血清、兔血清或BSA等封閉。